JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ427943.1
Database:	nt	Go to original record
Description:	Trichosanthes sp. HLQ090031 01 chloroplast, complete genome.
Taxon:	Trichosanthes sp. HLQ090031 01
Original size:	156871 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49264	49848	+	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaaaaaaagg gggtaggtgc
151   agagactcaa tggaagctgt tctaacaaat ggagttgact acattgcgtt
201   agtaaaggaa tgaatccttc tatcgaaact ccagaaaaga tgaaagataa
251   acgtattacg tactgaaata ctatttgatt aatgacaacc cgaatctcta
301   tttttttttt atataaatat aaaaaatgat atataaaaat gaacgaattg
351   ttctgaatcg attccaatat ctccaagttg aaaaaagaat cgaatattca
401   ttgatcaaat catttactcc atcatacata gtctgataga tcttttgaag
451   aactgattaa tcagatgcga atagaataaa gatagagtcc cattctacat
501   gtcaataccg acaaaaatga aatttatagt aagaggaaaa tccgtcgact
551   ttaaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details