JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ427942.1
Database:	nt	Go to original record
Description:	Trichosanthes sp. HLQ090035 01 chloroplast, complete genome.
Taxon:	Trichosanthes sp. HLQ090035 01
Original size:	156589 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49284	49871	+	588 bp

Amplicon:

588 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaaaaaaggg gtaggtgcag
151   agactcaatg gaagctgttc taacaaatag agttgactac gttgcggtag
201   taaaggaatg aatccttcta tcgaaactcc agaaaagatg aaagataaac
251   gtattacgta ctgaaatact atttgattaa tgacaaccca aatctctatt
301   tttttttttt tttatataaa tatcaaaaat gatatataaa aatgaacgaa
351   gtgttctgaa tcgattccaa tatctccaag ttgaaaaaag aatcgaatat
401   tcattgatca aatcatttac tccatcatac atagtctgat agatcttttg
451   aagaactgat taatcagatg cgaatagaat aaagatagag tcccattcta
501   catgtcaata ccgacaaaaa tgaaatttat agtaagagga aaatccgtcg
551   actttaaaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	588	569	2.65e-08

Match details