JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ427941.1
Database:	nt	Go to original record
Description:	Trichosanthes sp. HLQ090003 01 chloroplast, complete genome.
Taxon:	Trichosanthes sp. HLQ090003 01
Original size:	157200 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49934	50526	+	593 bp

Amplicon:

593 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaaaaaaaag ggtaggtgca
151   gagactcaat ggaagctgtt ctaacaaatg gagttgacta cgttgcgtta
201   gtaaaggaat gaatccttct atcgaaactc cagaaaagat gaaagataaa
251   cgtattacgt actgaaatac tatttgatta atgacaaccc gaatctcgat
301   tttttttttt tttttattta tataaaataa aaaatgatat ataaaaatga
351   acgaattgtt ctgaatcgat tccaatatct ccaagttgaa aaaagaatcg
401   aatattcatt gatcaaatca tttactccat catacatagt ctgatagatc
451   ttttgaagaa ctgattaatc agatgcgaat agaataaaga tagagtccca
501   ttctacatgt caataccgac aaaaatgaaa tttatagtaa gaggaaaatc
551   cgtcgacttt aaaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	593	574	2.67e-08

Match details