JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ427936.1
Database:	nt	Go to original record
Description:	Trichosanthes homophylla isolate PE 05012 chloroplast, complete genome.
Taxon:	Trichosanthes homophylla
Original size:	157332 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49757	50340	+	584 bp

Amplicon:

584 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaaaaaaagg gtaggtgcag
151   agactcaatg gaagctgttc taacaaatgg agttgactac gttgcgttag
201   taaaggaatg aatccttcta tcgaaactcc agaaaagatg aaagataaac
251   gtattacgta ctgaaatact atttgattaa tgacaacccg aatctctatt
301   tttttttttt tatttatata aaaaatgata tataaaaatg aacgaattgt
351   tctgaatcga ttccaatatc tccaagttga aaaaagaatc gaatattcat
401   tgatcaaatc atttactcca tcatacatag tctgatagat cagttgaaga
451   actgattaat cagatgcgaa tagaataaag atagagtccc attctacatg
501   tcaataccga caaaaatgaa atttatagta agaggaaaat ccgtcgactt
551   taaaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	584	565	2.63e-08

Match details