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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: ON087596.1
Database: nt Go to original record
Description: Oryza sativa Indica Group transgenic clone Chi10_RB_GSP2 Chi10_RB insertion sequence.
Taxon: Oryza sativa Indica Group
Original size: 772 bp

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Method: QL-ELE-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998).
Target:T-nos

View other 15527 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 1 103 282 + 180 bp
Amplicon: 180 bp 
1     aatcctggtt gccggtcttg cgatgattat catataattt ctgttgaatt
51    acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
101   tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
151   aaacaaaata tagcgcgcaa actaggataa

View other 1 match with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 95.0 20 bp 0 1 1 20 1.43e-06
primerB 100.0 20 bp 0 0 180 161 8.54e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.