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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: ON018824.1
Database: nt Go to original record
Description: Oryza sativa Indica Group transgenic clone OSM20_RB_AP2 OSM20_RB insertion sequence.
Taxon: Oryza sativa Indica Group
Original size: 735 bp

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Method: QL-ELE-00-009 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (Lipp et al.,2001).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 401 519 - 119 bp
Amplicon: 119 bp 
1     gcatgacgtt atttatgaga tgggttttct atgcctagag tcccgcaatt
51    atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
101   aattatcgcg cgcggtgtc
Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 24 bp 0 0 1 24 4.26e-11
primerB 100.0 24 bp 0 0 119 96 4.26e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.