JRC GMO-Amplicons | EURL GMFF

Record ID:	OP779244.1
Database:	nt	Go to original record
Description:	Impatiens huangyanensis chloroplast, complete genome.
Taxon:	Impatiens huangyanensis
Original size:	152228 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46206	46774	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt tggaaaacaa atccaaataa
151   agattccgaa acccaaaata gaaaaaggat aggtgcagag actcaatgga
201   agctgttcta acaaatggag ttgactgcgt tggtagagga attcctggat
251   cgaaacttca gaaagtatga aagaaaaaca tatatacata tgcataagaa
301   aataaatgac gatccaaaag cgtatccgtt ttttttagga aaaagtggtt
351   gtgaatcgat tccatattga ataaagaatc aaatattcat tggtcaaatc
401   attcactcca gactccatag tctgatagtt cctcggaaga actgattaat
451   cagacgagaa taaagataga gtcccattat acatgtcaat ccggacaaca
501   atgaaattta tagtaatagg aaaatccgtc gactttagaa atcgtgaggg
551   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details