JRC GMO-Amplicons | EURL GMFF

Record ID:	AF190401.1
Database:	nt	Go to original record
Description:	Eucalyptus pilularis tRNA-Leu and tRNA-Phe genes, partial sequence; chloroplast genes for chloroplast products.
Taxon:	Eucalyptus pilularis
Original size:	776 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	552	+	552 bp

Amplicon:

552 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgacaacttt caaattcaga gaaaccctgg aattaaagat
101   gggcaatcct gagccaaatc ctgttttacg aaaaccaaca aaacagtaag
151   ggttcataaa gcgagaataa aaaaaggata ggataggtgc agagactcaa
201   tgggagctgt tctaacaaat ggagttgacc acgttgcgtt ggtaaaggaa
251   tctttttctc gaaactcgag aaagggaaag gataaaccta tgtatatacg
301   tatacgtact gaatactatc tcaaatgatt aatgacgacg cgaatttttt
351   atatgaaaaa ggaaagaatc gaatattcat tgattaaatc atttactccg
401   tggtctgata aatcttttga agaactgatt aatcggacga gaataaagat
451   agagtcccat tctacatgtc aataccgaca acaatgaaat ttagagtaag
501   aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt ccctctatcc
551   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	552	533	2.48e-08

Match details