JRC GMO-Amplicons | EURL GMFF

Record ID:	AF190399.1
Database:	nt	Go to original record
Description:	Eucalyptus camaldulensis tRNA-Leu and tRNA-Phe genes, partial sequence; chloroplast genes for chloroplast products.
Taxon:	Eucalyptus camaldulensis
Original size:	808 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	580	+	580 bp

Amplicon:

580 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgacaacttt caaattcaga gaaaccctgg aattaaagat
101   gggcaatcct gagccaaatc ctgttttacg aaaaccaaca aaacaataag
151   ggttcataaa gcgagaataa aaaaaggata ggtgcagaga ctcaatggga
201   gctgttctaa caaatggagt tgaccacgtt gcgttggtaa aggaatcttt
251   ttctcgaaac tcgagaaagg gaaaggataa acctatgtat atacgtatac
301   gtactgaata ctatctcaaa tgattaatga cgacgcgaat tttttatatg
351   aaaaaggaaa gaattgttgt gaatcaattc taagttgaag aaagaatcga
401   atattcattg attaaatcat ttactccgtg gtctgataaa tcttttgaag
451   aactgattaa tcggacgaga ataaagatag agtcccattc tacatgtcaa
501   taccgacaac aatgaaattt agagtaagag gaaaatccgt cgactttaaa
551   aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	580	561	2.61e-08

Match details