JRC GMO-Amplicons | EURL GMFF

Record ID:	KM524102.1
Database:	nt	Go to original record
Description:	Sclerochloa dura voucher US:Soreng 8072 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Sclerochloa dura
Original size:	1692 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	803	1411	+	609 bp

Amplicon:

609 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacctgg ggttctcgaa
151   ctagaataca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg agttaattac gttgtgttgt tagtggaatt ccttctaaat
251   tcgagaagga agggctttat acatctaata aacacctata gatactgcca
301   tagcaaacga ttaatcacag aacccatatt ataatatagg ttctttattc
351   ttttttagaa tcaaatagaa atgattatga aataaaaatt cataattttt
401   ttagaattat tgtgaatcca ttcccatcga atatttagta atcaaatcct
451   tcaattcaaa gttttgaggt ctttaaaaaa gtagattaat cgaacgagga
501   caaagagaga gtcccattct acatgtcaat actgacaaca atgaaatttc
551   tagtaaaagg aaaatccgtc gactttataa gtcgtgaggg ttcaagtccc
601   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	609	590	2.74e-08

Match details