JRC GMO-Amplicons | EURL GMFF

Record ID:	KM524099.1
Database:	nt	Go to original record
Description:	Puccinellia pusilla voucher US:Soreng & Soreng 7360 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Puccinellia pusilla
Original size:	1713 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	813	1428	+	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacctgg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtggaat tccttctaaa
251   ttcgagaagg aagggcttta tacatctaat aaacacctat agtatagata
301   ctgccatagc aaacgattaa tcacagaacc catattataa tataggttct
351   ttattctttt ttagaatcaa atagaaatga ttatgaaata aaaaattcat
401   aattttttta gaattattgt gaatccattc ccatcgaata tttagtaatc
451   aaatccttcc attcaaagtt ttgaggtctt taaaaaagta gattaatcga
501   acgaggacaa agagagagtc ccattctaca tgtcaatact gacaacaatg
551   aaatttctag taaaaggaaa atccgtngac tttataagtt gtgagggttc
601   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details