JRC GMO-Amplicons | EURL GMFF

Record ID:	KM524085.1
Database:	nt	Go to original record
Description:	Phleum subulatum voucher CAN:Gillespie et al. 10367 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Phleum subulatum
Original size:	1770 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	826	1434	+	609 bp

Amplicon:

609 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatgaaa
51    cctgctaagt ggtaacttcc aaattcagag aaaccctgga attaaaaaag
101   ggcaatcctg agccaaatcc gtgttttgag aagggggttc tcgaactaga
151   atacaaagga aaaggatagg tgcagagact caatggaagc tgttctaacg
201   aatcgagtta attacgttgt gttgttagtg ttagtggaat tccttctaaa
251   ttcgagaaag aagggcttta tacatctaat aaacacgtat agatactgat
301   agcaaacgat taatcacaga acccatatta taatataggt tctttattct
351   tttttagaat gaaatagaaa tgattatgaa ataaaaaatt cagaattttt
401   ttagaattat tgtgaatcca ttccaatcga atatttagta atcaaatcct
451   tcaattcaaa gttttgaggt ctttaaaaaa gtggattaat cggacgagga
501   caaagagaga gtcccattct acatgtcaat actgacaaca atgaaatttc
551   tagtaaaagg aaaatccgtc gactttataa gtcgtgaggg ttcaagtccc
601   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	609	590	2.74e-08

Match details