JRC GMO-Amplicons | EURL GMFF

Record ID:	KM524082.1
Database:	nt	Go to original record
Description:	Phleum phleoides voucher US:Soreng et al. 7881 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Phleum phleoides
Original size:	1767 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	828	1431	+	604 bp

Amplicon:

604 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaagggggtt ctcgaactag
151   aatacaaagg aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   gaatcgagtt aattacgttg tgttgttagt ggaattcctt ctaaattcga
251   gaaagaaggg ctttatacat ctaataaaca cgtatagata ctgatagcaa
301   acgattaatc acagaaccca tattataata taggttcttt attctttttt
351   agaatgaaat agaaatgatt atgaaataaa aaattctgaa tttttttaga
401   attattgtga atccattcca atcgaatatt tagtaatcaa atccttcaat
451   tcaaagtttt gaggtcttta aaaaagtgga ttaatcggac gaggacaaag
501   agagagtccc attctacatg tcaatactga caacaatgaa atttctagta
551   aaaggaaaat ccgtcgactt tataagtcgt gagggttcaa gtccctctat
601   cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	604	585	2.72e-08

Match details