JRC GMO-Amplicons | EURL GMFF

Record ID:	KM524081.1
Database:	nt	Go to original record
Description:	Phleum montanum voucher CAN:Gillespie et al. 10614-2 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Phleum montanum
Original size:	1768 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	828	1432	+	605 bp

Amplicon:

605 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaagggggtt ctcgaactag
151   aatacaaagg aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   gaatcgagtt aattacgttg tgttgttagt ggaattcctt ctaaattcga
251   gaaagaaggg ctttatacat ctaataaaca cgtatagata ctgaatagca
301   aacgattaat cacagaaccc atattataat ataggttctt tattcttttt
351   tagaatgaaa tagaaatgat tatgaaataa aaaattcaga atttttttag
401   aattattgtg aatccattcc aatcgaatat ttagtaatca aatccttcaa
451   ttcaaagttt tgaggtcttt aaaaaagtgg attaatcgga cgaggacaaa
501   gagagagtcc cattctacat gtcaatactg acaacaatga aatttctagt
551   aaaaggaaaa tccgtcgact ttataagtcg tgagggttca agtccctcta
601   tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	605	586	2.73e-08

Match details