JRC GMO-Amplicons | EURL GMFF

Record ID:	KM524079.1
Database:	nt	Go to original record
Description:	Phleum alpinum voucher CAN:Saarela 1224 (07-JMS-1224) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Phleum alpinum
Original size:	1785 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	835	1445	+	611 bp

Amplicon:

611 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaagggggtt ctcgaactag
151   aatacaaagg aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   gaatcgagtt aattacgttg tgttgttagt gttagtggga ttccttctaa
251   attcgagaaa gaagggcttt atacatctaa taaacacgta tagatactga
301   atagcaaacg attaatcaca gaacccatat tataatatag gttctttatt
351   cttttttaga atgaaataga aatgattatg aaataaaaaa ttcagaattt
401   ttttagaatt attgtgaatc cattccaatc gaatatttag taatcaaatc
451   cttcaattca aagttttgag gtctttaaaa aagtggatta atcggacgag
501   gacaaagaga gagtcccatt ctacatgtca atactgacaa caatgaaatt
551   tctagtaaaa ggaaaatccg tcgactttat aagtcgtgag ggttcaagtc
601   cctctatccc c

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	611	592	2.75e-08

Match details