JRC GMO-Amplicons | EURL GMFF

Record ID:	KM524074.1
Database:	nt	Go to original record
Description:	Milium vernale voucher CAN:Gillespie et al. 10445 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Milium vernale
Original size:	1711 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	816	1429	+	614 bp

Amplicon:

614 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tgttaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta ccttgtgttg ttagtggaat tccttctaaa
251   ttcgagaaag aagggcttta tacatctaat aaacacatat agatgcataa
301   caaacgatta atcacagaat ccatattata attataatat aggttcttta
351   ttctttttta gaatgaaata gaaataatta ttaaataaaa aattctgaat
401   ttttttagaa ttattgtgaa tccattccaa tcgaatattt agtaatcaaa
451   tccttgcaat tcaaagtttt gaggtcttta aaaaagtgga ttaatcggac
501   gaggacaaag agagagtccc attctacatg tcaatactga caacaatgaa
551   atttctagta aaaggaaaat ccgtcgactt tataagtcgt gagggttcaa
601   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	614	595	2.77e-08

Match details