JRC GMO-Amplicons | EURL GMFF

Record ID:	KM524073.1
Database:	nt	Go to original record
Description:	Milium schmidtianum voucher US:Soreng et al. 8019 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Milium schmidtianum
Original size:	1773 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	860	1473	+	614 bp

Amplicon:

614 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaaga gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaac ggaagctgtt
201   ctaacgaatc gagttaatta tgttgtgttg ttagtggaat tccttctaac
251   ttcgagaaag aagggcttta tacatctaat aaacacgtat agatacatag
301   caaacgatta atcacagaat ccatattata atataggttc tttattcttt
351   tttagaatga aatttgaaat ataaattctt attaaataaa aaattcagaa
401   tttttttaga attattttga atccattcca atcgaatatt tagtaatcaa
451   atccttcaat tcaaagtttt gaggtcttta aaaaagtgga ttaattggac
501   gaggacaaag agagagtccc attctacatg tcaatactga caacaatgaa
551   atttctagta aaaggaaaat ccgtcaactt tataagtcgg gagggttcaa
601   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	614	595	2.77e-08

Match details