JRC GMO-Amplicons | EURL GMFF

Record ID:	KM524063.1
Database:	nt	Go to original record
Description:	Colpodium versicolor voucher US:Soreng et al. 8012 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Colpodium versicolor
Original size:	1740 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	790	1408	+	619 bp

Amplicon:

619 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaaccagg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg tttgttagtc aaattccttc
251   taaattcgag aaggaagggc tttatacatc taataaacac ctatagatac
301   tgacatagca aacgattaat cacagaaccc atattaaaat ataggttctt
351   tattcttttt tagaatgaaa tagaaatgat tatgaaataa aaaattcata
401   atttttaaaa aattattgtg aatccattcc catcgaatat ttagtaatca
451   aatccttcaa tcaattcaaa gttttgaggt ctttaaaaaa gtagattaat
501   cggacgagga caaagagaga gtcccattct acatgtcaat actgacaaca
551   atgaaatttc tagtaaaagg aaaatccgtc gactttataa gtcgtgaggg
601   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	619	600	2.79e-08

Match details