JRC GMO-Amplicons | EURL GMFF

Record ID:	KM524062.1
Database:	nt	Go to original record
Description:	Catabrosella variegata subsp. variegata voucher US:Soreng et al. 8040 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Catabrosella variegata subsp. variegata
Original size:	1640 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	751	1357	+	607 bp

Amplicon:

607 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaagggggtt ctagaactag
151   aatacaaagg aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   gaatcgagtt aatttgtgtt gttagtggaa ttccttctaa attcgagaag
251   gaagggcttt atacatctaa taaataccta tagatactgc catagcaaac
301   gattaatcac agaacccata ttataatata atataggttt tttattcttt
351   tttagaatga aatagaaatg attatgaaat aaaaaattca taattttttt
401   agaattattg tgaatccatt cccatcgaat atttagtaat caaatccttc
451   aattcaaagt tttgaggtct ttaaaaaagg agattaatcg aacgaggaca
501   aagagagagt cccattctac atgtcaatac tgacaacaat gaaatttcta
551   gtaaaaggaa aatccgtcga ctttataagt cgtgagggtt caagtccctc
601   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	607	588	2.73e-08

Match details