JRC GMO-Amplicons | EURL GMFF

Record ID:	KM524045.1
Database:	nt	Go to original record
Description:	Alopecurus lanatus voucher CAN:Gillespie et al. 10408-1 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Alopecurus lanatus
Original size:	1771 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	821	1435	+	615 bp

Amplicon:

615 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaagggggtt ctcgcactag
151   aatacaaagg aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   gaatcgagtt aattatgttg tgttgttagt ggaattcctt ctaaattcga
251   gaaagaaggg ctttatacat ctaataaaca cgtatagata ctgacatagc
301   aaacgattaa tcacagaacc tatattataa tatatggttc tttattcttt
351   tttagaatga aatagaaatg attatgaaat aaaaaattca taattttttt
401   tttagaatta ttgtgaatac attccaatcg aatatttagt aatcaaatgc
451   ttcaattcaa agttttgagg tctttaattt aaaaaagtgg attaatcgga
501   cgaggacaaa gagagagtcc cattctacat gtcaatactg acaacaatga
551   aatttctagt aaaaggaaaa tccgtcgact ttataagtcg tgagggttca
601   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	615	596	2.77e-08

Match details