JRC GMO-Amplicons | EURL GMFF

Record ID:	KM524044.1
Database:	nt	Go to original record
Description:	Alopecurus japonicus voucher MO:Tsugaru et al. 1495 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Alopecurus japonicus
Original size:	1740 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	843	1404	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtattttga gaaaacctgg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtattg ttagtggaat tccttctaaa
251   ttcgagaaag aagggcttta tacatgtaat aaacacgtat agatgacata
301   gcaaacgatt aatcacagaa cctatattat aatatatggt tctttattct
351   tttttagaat tattgtgaat acattccaat cgaatattta gtaatcaaat
401   gcttcaattc aaagttttga ggtctttaaa aaagtggatt aatcggacga
451   ggacaaagag agagtcccat tctacatgtc aatactgaca acaatgaaat
501   ttctagtaaa aggaaaatcc gtcgacttta taagtcgtga gggttcaagt
551   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details