JRC GMO-Amplicons | EURL GMFF

Record ID:	KM524039.1
Database:	nt	Go to original record
Description:	Alopecurus davisii voucher CAN:Gillespie et al. 10459-1 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Alopecurus davisii
Original size:	1769 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	820	1433	+	614 bp

Amplicon:

614 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaagggggtt ctcgaactag
151   aatacaaagg aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   gaatcgagtt aattatgttg tgttgttagt ggaattcctt ctaaattcga
251   gaaagaaggg ctttatacat ctaataaaca cgtatagata ctgacatagc
301   aaacgattaa tcacagaacc tatattataa tatatggttc tttattcttt
351   tttagaatga aatagaaatg attatgaaat aaaaaattca taattttttt
401   ttagaattat tgtgaataca ttccaatcga atatttagta atcaaatgct
451   tcaattcaaa gttttgaggt ctttaattta aaaaagtgga ttaatcggac
501   gaggacaaag agagagtccc attctacatg tcaatactga caacaatgaa
551   atttctagta aaaggaaaat ccgtcgactt tataagtcgt gagggttcaa
601   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	614	595	2.77e-08

Match details