JRC GMO-Amplicons | EURL GMFF

Record ID:	OP186460.1
Database:	nt	Go to original record
Description:	Elsholtzia fruticosa voucher H3050369 chloroplast, complete genome.
Taxon:	Elsholtzia fruticosa
Original size:	151583 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46108	46670	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacca aaacaaaggt
151   tcaaaaaacg aaaaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttgactgcg ctgggtagag gaatctttcc atggaaactt
251   tagaaaggat gaagaataaa cgcatctagt gaatactata tcaaatggtt
301   aatgatgggc ccaaatctgt ttttttattt taatatgaaa aatggaaaaa
351   tttgtgtgaa ttgattccac gttgaagaaa aaattgaata ttcatcacct
401   cattcactcc atagtccgat acatctttta aagaattgat taatcgaacg
451   agaataaaga tagagtccca ttctacatgt caataccggc aacaatgaaa
501   tttatagtaa gaggaaaatc cgtcgacttt caaaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details