JRC GMO-Amplicons | EURL GMFF

Record ID:	OP186458.1
Database:	nt	Go to original record
Description:	Elsholtzia densa voucher H3050021 chloroplast, complete genome.
Taxon:	Elsholtzia densa
Original size:	149081 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45229	45784	+	556 bp

Amplicon:

556 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccccgg aattaataaa
101   aacgggcaat cctgagccaa atcctatttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa ggggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctacgctggt agaggaatct ttccatgcaa actttagaaa
251   ggatgaagga taaacgcatc tagtgaatac tatatcaaat gattaatgat
301   gggcccgaat ctgttttttt agtttaatat gaaaatggaa aaatttgtgt
351   gaattgattc cacgttgaag aaaaatttga atattcatca cctcgttcac
401   tccatagtcc gatatatctt ttaaagaact gattaatcgg acaagaataa
451   agatagagtc ccattctaca cgtcaatacc ggcaacaatg aaatttctag
501   taagaggaaa atccgtcgac tttaaaaatc gtgagggttc aagtccctct
551   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	556	537	2.50e-08

Match details