JRC GMO-Amplicons | EURL GMFF

Record ID:	OP169593.1
Database:	nt	Go to original record
Description:	Eremophila capricornica isolate BMR01824 chloroplast, complete genome.
Taxon:	Eremophila capricornica
Original size:	151581 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48157	48713	+	557 bp

Amplicon:

557 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   agcgaaaaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gactgcgttg gtagaggaat ctttccatcg aaacttcaga
251   aaggataaag gtatatattg aatactatct caaatgatta atgatggccc
301   taatctgtat ctgtattttt ttatatgaaa aatggaagaa ttggtatgaa
351   gtgattccac attgaagaaa gaatcaaata ttccttcatc aaatcattca
401   ctccacagtc tgatagttct tttaaagaac tgattaatcg gacaagaata
451   aagatagagt cccattctac atgtcaatat tggcaacaat gcaatttata
501   gtaagaggaa aatccgtcga ctttaaaagt cgtgagggtt caagtccctc
551   tatcccc

View other 28 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	557	538	2.51e-08

Match details