JRC GMO-Amplicons | EURL GMFF

Record ID:	OP022558.1
Database:	nt	Go to original record
Description:	Impatiens morsei isolate 2 chloroplast, complete genome.
Taxon:	Impatiens morsei
Original size:	148277 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46391	46919	+	529 bp

Amplicon:

529 bp

1     cgaaatcggt agacgctacg gacttaattg gagtgagcct tggtatggaa
51    acctactaag cgataacttt caaattcaga gaaaccctgg aattaaatta
101   ataaaaatgg gcaatcctga gccaaatcct gtttttggaa aacaaatcca
151   aataaagatt ccgaaaccaa aaatagaaga aggataggtg cagagactca
201   acggaagctg ttctaacaaa tggagttgac tgcgttggta gaggaatccg
251   tggatcgaaa cttcagaaag tatgaaagaa aaacatatat acatatgcat
301   atatgctgaa atagtatatc aatactatat caaaagaaaa tgaatgacaa
351   tccaatcatt cactccgtac tccctaatgt gatagttcct ctgaagaact
401   gattaatcag acgagaataa agatagagtc ccattataga tgtcaatccc
451   gacaacaatg aaatttatag taataggaaa atccgtcgac tttagaaaaa
501   atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.38e-08
primerB	100.0	20 bp	0	0	529	510	2.38e-08

Match details