JRC GMO-Amplicons | EURL GMFF

Record ID:	ON186542.1
Database:	nt	Go to original record
Description:	Impatiens chlorosepala chloroplast, complete genome.
Taxon:	Impatiens chlorosepala
Original size:	152917 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46921	47485	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatggacaat cctgagccaa atcctgtttt tggaaaaaaa atccaaataa
151   agattccgaa accaaaaata gaaaaaggat aggtgcagag actcaatgga
201   agctgttcta acaaatggag ttgactgcgt tggtagagga attcgtggat
251   cgaaacttca gaaagtagga aagaaaaaca tatatacata tgcataagaa
301   aatgaatgac gatccaaaag cgtatccgtt tttttttagg aaaaaaaatg
351   gttgtgaatc gattccatat tgaatatcga atcaaatatt cattggtcaa
401   atcattcatt ccatagtctg atagttcctc tgaagaactg attaatcaga
451   cgagaataaa gatagagtcc cattatacat gtcaatcccg acaacaatga
501   aatttatagt aataggaaaa tccgtcgact ttagaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details