JRC GMO-Amplicons | EURL GMFF

Record ID:	OX415257.1
Database:	nt	Go to original record
Description:	Linaria vulgaris genome assembly, organelle: plastid:chloroplast
Taxon:	Linaria vulgaris
Original size:	156708 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	51373	51931	+	559 bp

Amplicon:

559 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   agcaaaaaag ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttgact atgttggtag aggaatcttt ccatcaaaac ttcagaaagg
251   atgaaggata aatgtatcta ttgaaaacta tatcaaatga ttaatgatgg
301   cccaaatctg tatctgtttt ttttatataa aaaatggaag aattgatgtg
351   aattgattcc atattgaaga aagaatcaaa tattcattca tcaaatcatt
401   cactccacag tctggtagtt cttttaaaga actgattaat cggacgagaa
451   taaagataga gtcccattct acatgtcaat accggcaaca attaaattta
501   tagtaagagg aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc
551   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	559	540	2.52e-08

Match details