JRC GMO-Amplicons | EURL GMFF

Record ID:	OX415247.1
Database:	nt	Go to original record
Description:	Sherardia arvensis genome assembly, organelle: plastid:chloroplast
Taxon:	Sherardia arvensis
Original size:	152730 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47614	48196	+	583 bp

Amplicon:

583 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acctgctgag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aggttaaaaa
151   ggggataggt gcagagactc aatggaagct gttctaacaa atggagttgg
201   ctgtgttagt cgagaattct ttccatcaaa aattcagaaa atacaagtat
251   taagtgaagg ataaacatat atactatcca tacgcagtga atagtatatt
301   ataatgagta atgacaaccc caatgaatct ctactttttc ataagaaaaa
351   atatagaatt gtagttgttg ttaaaatatt atacgtttaa gaaagaatcg
401   aatattcatt tatcaaatga ctcactccac agtctgatag atctttttac
451   gaactgatta attggacgag aataaagata gagtcccttt tctacatgtc
501   aatgccggca gcaatgaaat ttatagttaa taggaaaatc cgtcgacttt
551   taaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	583	564	2.63e-08

Match details