JRC GMO-Amplicons | EURL GMFF

Record ID:	OM176669.1
Database:	nt	Go to original record
Description:	Boeica sp. LF-2023a chloroplast, complete genome.
Taxon:	Boeica sp. LF-2023a
Original size:	154178 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48229	48772	+	544 bp

Amplicon:

544 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctactaag tgaagacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atccttgttt tctcaaaaca agcgttcaga
151   gagcgaaaaa gaggataggt gcagagactc aatggaagct gttctaacaa
201   acggagttga ctgcgttggt agaggaatcg aaacttcaga aaggatgaag
251   gataaacgta tctattgaat actattgatg attaataatg gcccgaatct
301   gcatctgggt ttttgtatat gaaaaatgga agaattggtg tgaattgatt
351   tcacattgaa tcaaatattc attgatcaaa tgattcactc catagtccga
401   tagatctctt aaagaactta ttattcggac gagaataaag atagagtccc
451   gttctacatg tcaataccgg caacaatgaa acttatagta agaggaaaat
501   ccgtcgactt taaaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.45e-08
primerB	100.0	20 bp	0	0	544	525	2.45e-08

Match details