JRC GMO-Amplicons | EURL GMFF

Record ID:	OP076942.1
Database:	nt	Go to original record
Description:	Smilax china voucher K. H. Wong 154 chloroplast, complete genome.
Taxon:	Smilax china
Original size:	158269 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46766	47379	+	614 bp

Amplicon:

614 bp

1     cgaaatcggt agacgctacg gacttgatta gcttgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccccgg aattcaaaat
101   gggcaatcct gagccaaatc tttattttga taaaataggg gtttaattta
151   taaaactaga atcaaaaagg gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatg gagttgacta cgttacgttg cgttggtagc tggaatccct
251   ctatcaaaat tacagatagg atggccctat atacgtatat atacatactg
301   acatatcaaa cgattgatta attatgaata tatttatata tatatatata
351   tatatgatat gaatatatga atatgaaaaa aaaaattgag agttattgtg
401   aatctattcc aatcgaagtt gaagaaagaa tcgaatattc agttatcaaa
451   ttattcattc cagagtctga aaaatctttt gaaaaactga ttaattggac
501   gagaataaag agagagtccc attctacatg tcaatatcga caacaatgaa
551   atttatagta aaaggaaaat ccgtcgactt tagaagtcgt gagggttcaa
601   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	614	595	2.77e-08

Match details