JRC GMO-Amplicons | EURL GMFF

Record ID:	ON815616.1
Database:	nt	Go to original record
Description:	Metastachydium sagittatum isolate J81641 voucher KUN-16CS12196 plastid, complete genome.
Taxon:	Phlomoides sagittata
Original size:	151784 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46719	47279	+	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttaatta gaattaaatt gagccttagt
51    gtgggaaacc tactaagtga taactttcaa attcagagaa accccggaat
101   taagaaaaat gggcgatcct gatccaaatc ctgtttttca aaacaaaggt
151   tcaaaaaacg aactaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttgactgcg ttggtcgagg aatcctttct acggaaactt
251   cagaaaggat gaaggataaa cgtatctatc gaatactata tcaaatgatt
301   aatgatggcc cgaatccgta tttttaatat gaaaaataga agaattggtg
351   tgaattgatt ctatattgaa gaaaaaatcg aatattcatt catcaaatca
401   ttcactccat aatccgatag atcttttaaa gaactgatta atcggacgag
451   aataaagata gagtcccatt ctacatgtca atactggcaa caatgaaatt
501   tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc
551   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details