JRC GMO-Amplicons | EURL GMFF

Record ID:	ON815615.1
Database:	nt	Go to original record
Description:	Betonica officinalis isolate LA2211 voucher KUN-EM464 plastid, complete genome.
Taxon:	Betonica officinalis
Original size:	151580 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46593	47154	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaatta taatgaaatt gagccttagt
51    gtgggaaacc cactaagtga taactttcaa attcagagaa accccggaat
101   taataaaaat gggcgatcct gatccaaatc ctgtttttca aaacaaaggt
151   tcaaaaaacg aaataaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttggctgcg ttggtagagg aatcctttct atggaaactt
251   cagaaagtat gaaggataaa cgtatctatc gaatactata tcaaatgatt
301   aatgatggcc cgaatttgta ttttttaata tgaaaaatag aagaattggt
351   gtgaattgat tctatattga agaaaaaatg gaatattcat tcatcaaatc
401   attcactcca tagtccgata gatcttttaa agaactgatt aatcggacga
451   gaataaagat agagtcccat tctacatgtc aatactggca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt
551   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details