JRC GMO-Amplicons | EURL GMFF

Record ID:	ON764436.1
Database:	nt	Go to original record
Description:	Amana sp. MW-2022b isolate 2 chloroplast, complete genome.
Taxon:	Amana sp. MW-2022b
Original size:	151522 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45369	45937	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttttttttt ttttcaaaaa aaaagatagg
151   tgcagagact caatggaagc tgttctaaca aatggagttg attacgttac
201   atgcgctagt agccggaatc cttctatcaa aattattgaa aagatacccg
251   ccctatatat gtagatatac atactcacat agtaaacgat tgattaatcg
301   cagcccaaat ccattatata tgaaaaattt aagaatcatt gtgaatctat
351   tccattcgat attccaatcg aagttggagg aagaatcgaa tattaaatga
401   tcaaattatt cattccaaag tctgatagat cttttgaaaa accgattatt
451   cgaacgagaa taaagagaga gtcccattct acgtgtcaat accgacaaca
501   atgaaattta tagtaaaagg aaaatccgtc gactttaaaa gtcgtgaggg
551   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details