JRC GMO-Amplicons | EURL GMFF

Record ID:	ON756033.1
Database:	nt	Go to original record
Description:	Meconopsis pseudohorridula chloroplast, complete genome.
Taxon:	Meconopsis pseudohorridula
Original size:	153989 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46317	46912	+	596 bp

Amplicon:

596 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcag aaaaaattaa gggatttctg
151   aaagcgagaa taaaaaaagg ataggtgcag agactcaatg gaagctgttc
201   taatgaatgg agttgactgc gttggtagag gaatccttct attaaatttt
251   aatttaaact ccataaagaa tgagggatga acctatatac gatacgtacg
301   tatacgtact gaaatatcaa agattctatt aatgccgccc taaatatatt
351   tttttacata aaaaaaggaa taatttttgt gaatctattc caagttgaag
401   gaagaatcga aaattcagtg atcaaaccat tcactccata gtctgataga
451   tcttttgacg aactgattaa tcggacgaga ataaagatag agtcccgttc
501   tacatgtcaa tatcaatacc gacaacaatg aaatttatag taagaggaaa
551   atccgtcgac tttataaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	596	577	2.68e-08

Match details