JRC GMO-Amplicons | EURL GMFF

Record ID:	MW392090.1
Database:	nt	Go to original record
Description:	Plumeria jamaicensis chloroplast, partial genome.
Taxon:	Plumeria jamaicensis
Original size:	141465 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47996	48561	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataat
101   aaagggcaat cctgagccaa atcctgtttt tcacaaacaa aggttcagaa
151   aacgaaaaca aggataggtg cagagactca acggaagcta ttctaacaaa
201   tggagttggc cgcgttggta gagaaatctt tccgtcgaaa attcagaaag
251   gatgaagtat aaacgtatat acatacgtat tgaatactat atcaaatgat
301   taatgccaac ccgaatgaat ctgtattttt ttctataaaa actggaagaa
351   ttggtgtgat tcgattcttt cttcaatgtg gaatccaata ttcattgatc
401   aaatgattca ctccacagtc tgtagatctt ttcaagaact gattaatcgg
451   acgagaataa agatagagtc ccgttctaca tgtcaatgct ggcaacaatg
501   aaatttatag taagaggaaa atccgtcgac ttaaaaaatc gtgagggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details