JRC GMO-Amplicons | EURL GMFF

Record ID:	ON464732.1
Database:	nt	Go to original record
Description:	Elsholtzia minima chloroplast, complete genome.
Taxon:	Elsholtzia minima
Original size:	151160 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45866	46436	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aacgggcaat cctgagccaa atcctgtttt ctcaaaacca aaacaaaggt
151   tcaaaaaacg aaaaaaaaaa agggataggt gcagagactc aatggaagct
201   gttctaacaa atggagttga ctgcgctggg tagaggaatc tttccatgga
251   aactttagaa aggatgaaga ataaacgcat ctagtgaata ctatatcaaa
301   tggttaatga taggcccaaa tctgtttttt ttttatttta atataaaaaa
351   tggaaaaatt tgtgtgaatt gattccacgt tgaagaaaaa attgaatatt
401   catcacctca ttcactccat agtccgatac atcttttaaa gaattgatta
451   atcgaacgag aataaagata gagtcccatt ctacatgtca ataccggcaa
501   caatgaaatt tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag
551   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details