JRC GMO-Amplicons | EURL GMFF

Record ID:	ON456287.1
Database:	nt	Go to original record
Description:	Primulina hedyotidea chloroplast, complete genome.
Taxon:	Primulina hedyotidea
Original size:	153289 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47540	48092	+	553 bp

Amplicon:

553 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaaa gtgaagactt tcaaattcag agaaaccctg gaattaataa
101   aaatgggcaa tcctgagcca aatccttgtt ttctcaaaac aagggttcag
151   agagcgaaaa agaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg accacgttgg tagaagaatt gaaacttcag aaaggatgaa
251   ggataaacgt atctattgaa tactattgat gattaataat ggcccgaatc
301   tgtatctggg tattttctat atgaaaaata gaagaattgg tgtgaattga
351   tttcacattg ataaaagaat cgaatattta ttagtcaaat cattcactcc
401   acagtccgat agatctctta aagatcttag tattcggacg agaataaaga
451   tagagtcccg ttctacatgt caataccggc aacaatgaaa cttatagtaa
501   gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag tccctctatc
551   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	553	534	2.49e-08

Match details