JRC GMO-Amplicons | EURL GMFF

Record ID:	MW279192.1
Database:	nt	Go to original record
Description:	Amana sp. 12 QZ-2022 clone X01 chloroplast, complete genome.
Taxon:	Amana sp. 12 QZ-2022
Original size:	151287 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45129	45696	+	568 bp

Amplicon:

568 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttttttttt tttcaaaaaa aaggataggt
151   gcagagactc aatggaagct gttctaacaa atggagttga ttacgttaca
201   tgcgctagta gccggaatcc ttctatcaaa attattgaaa agatacccgc
251   cctatatatg tagatataca tactcacata gtaaacgatt gattaatcgc
301   agcccaaatc cattatatat gaaaaattta agaatcattg tgaatctatt
351   ccattcgata ttccaatcga agttggagga agaatcgaat attaaatgat
401   caaattattc attccaaagt ctgatagatc ttttgaaaaa ccgattattc
451   gaacgagaat aaagagagag tcccattcta cgtgtcaata ccgacaacaa
501   tgaaatttat agtaaaagga aaatccgtcg actttaaaag tcgtgagggt
551   tcaagtccct ctatcccc

View other 7 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	568	549	2.56e-08

Match details