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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: HF949851.1
Database: nt Go to original record
Description: Boechera divaricarpa GSS, clone B59-01-F_K03
Taxon: Boechera divaricarpa
Original size: 1219 bp

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Method: QL-ELE-00-004 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Lipp et al.,2001).
Target:CaMV P-35S

View other 10536 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 2 519 641 + 123 bp
Amplicon: 123 bp 
1     cacgtcttca aaagcaagtg gattgatgtg acatctccac tgacgtaagg
51    gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
101   aagttcattt catttgggag agg
Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 95.2 21 bp 0 1 1 21 3.08e-07
primerB 96.0 25 bp 0 1 123 99 2.17e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.