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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: HF949525.1
Database: nt Go to original record
Description: Boechera divaricarpa GSS, clone B59-01-R_M13
Taxon: Boechera divaricarpa
Original size: 1181 bp

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Method: QL-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (BVL L 00.00-31, 1998).
Target:CaMV P-35S

View other 10854 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 511 706 - 196 bp
Amplicon: 196 bp 
1     gctcttacaa atgccatcat ggcgataaag gaaaggctat cattcaagat
51    ctctctgccg acagtggtcc caaagatgga ccccccaccc acgaggagca
101   tcggggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
151   tgtgacatct ccactgacgt aagggatgac gcacaatccc actatc

View other 1 match with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 94.7 19 bp 1 0 1 19 1.45e-06
primerB 100.0 20 bp 0 0 196 177 9.33e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.