JRC GMO-Amplicons | EURL GMFF

Record ID:	KP699016.1
Database:	nt	Go to original record
Description:	Vicia amoena isolate L-F_SY3 tRNA-Leu (trnL), trnL-F intergenic spacer, partial sequence; chloroplast.
Taxon:	Vicia amoena
Original size:	684 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	526	+	526 bp

Amplicon:

526 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttaccaag tgaaaacttt caaattcaga gaaaccctgg aattcaaaat
101   gggcaatcct gagccaaatc cttctttccg aaaacaaaaa aagaaaagtt
151   cagaaagttc aaatcaaaaa aggataggtg cagagactca atggaagctg
201   ttctaacaaa tggagttgac aacattcaat tgattaatga agatttctaa
251   cttctatttg taaattgtca atattttgtt ttgattctat cacaattgaa
301   acattagaat caaatcaatt acaactggaa gaaaaaatgg tgaatattca
351   ttgatccaat cagtcactcc actataatct gatggatctt ttgaatcact
401   gattaatcag acgagaataa agatagagtc ccattctaca tgtcaatacc
451   gacatcaatg aaattttgag taagaggaaa atccgtcgac tttagaaatc
501   gtgagggttc aagtccctct atcccc

View other 13 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.37e-08
primerB	100.0	20 bp	0	0	526	507	2.37e-08

Match details