JRC GMO-Amplicons | EURL GMFF

Record ID:	KP699011.1
Database:	nt	Go to original record
Description:	Vicia amurensis isolate L-F_HLJ3 tRNA-Leu (trnL), trnL-F intergenic spacer, partial sequence; chloroplast.
Taxon:	Vicia amurensis
Original size:	679 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	521	+	521 bp

Amplicon:

521 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttaccaag tgaaaacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc cttctttccg aaaacaaaaa aagaaaagtt
151   cagaaagttc aaatcaaaaa aggataggtg cagagactca atggaagctg
201   ttctaacaaa tggagttgac aacattcaat tgattaatga agatttcgaa
251   cttctatttg taaattgtca atattttgtt ttgattctat cacaattgaa
301   acattagaat caattacaac tggaagaaaa aatgttgaat attcattgat
351   ccaatcagtc actccactat aatctgatgg atcttttaaa tcactgatta
401   atcagacgag aataaagata gagtcccatt ctacatgtca ataccgacat
451   caatgaaatt ttgagtaaga ggaaaatccg tcgactttag aaatcgtgag
501   ggttcaagtc cctctatccc c

View other 4 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.34e-08
primerB	100.0	20 bp	0	0	521	502	2.34e-08

Match details