JRC GMO-Amplicons | EURL GMFF

Record ID:	ON408231.1
Database:	nt	Go to original record
Description:	Zygophyllum xanthoxylon plastid, complete genome.
Taxon:	Zygophyllum xanthoxylum
Original size:	105802 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47451	47981	+	531 bp

Amplicon:

531 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acatactaag tgatcacttt caaattcaga gaaaccctgg aattagaaat
101   gggcaatcct gagccaaatc ctgttttcaa aaaaaaagaa ttcaattaaa
151   agaatcaaaa aaggataggt gcagagactc aatggaagct gttctaaaaa
201   atggagttga ctgctgcatt acattagtca agtcaagtaa tccttgcatc
251   gaaattttca aaaagataaa ggataacctt tctttctata aaactataaa
301   aaaaaagact ttatgaatca attcgaagtt gaagaaagaa tcaaatatga
351   tttgataaaa tcatttactc caaagtctga tatatctttt tcaaaatgga
401   ttagtcagat gagaataaag atagagtccc attctatatg tcagtattta
451   caattgacaa caatgaaatt tatagtgaga ggaaaatccg tcgattttat
501   aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.39e-08
primerB	100.0	20 bp	0	0	531	512	2.39e-08

Match details