JRC GMO-Amplicons | EURL GMFF

Record ID:	ON149877.1
Database:	nt	Go to original record
Description:	Caryopteris forrestii plastid, complete genome.
Taxon:	Caryopteris forrestii
Original size:	151854 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46991	47553	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctgctaag tgataacttt caaattcaga gaaaccccgg aactaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaccaa aggttcaaaa
151   aacgaaaaga aaaaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacaaatg gagttgactg tgctggtaga ggaatctttc catggaaact
251   tcagaaagga tgaaagataa atgtatctat tgaatactat atcaaatgat
301   taatgatggc ccgaatctgt attttttaat atgaaaaatg gaagaattgg
351   tgtgaattga ttccacattg aagaaaaaat cgaatattca ttcatgaaat
401   cattcactcc atagtctgat cgatcttttc aagaactgat taatcggacg
451   agaataaaga tagagtcccg ttctacatgt caataacggc aacaatgaaa
501   tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details