JRC GMO-Amplicons | EURL GMFF

Record ID:	OM912812.1
Database:	nt	Go to original record
Description:	Clerodendrum thomsoniae chloroplast, complete genome.
Taxon:	Clerodendrum thomsoniae
Original size:	151053 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46493	47052	+	560 bp

Amplicon:

560 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctgctaag tgatgacttt caaattcaga gaaaccccgg aactaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggtttaaaa
151   aacgaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttgact gcgctggtag aggaatcttt ccatagaaac ttcagaaagg
251   atgaaggata aatggatcta ttgaatacta tatcgaaatc gaatgattaa
301   tgatggcccg aatctgtatt ttttaatatg aaaaatggaa gaattggtgt
351   gaattgattc cacattgaat aaaaaatcga atattcattc atcaaatcat
401   tcactccaca gtccgataga tcttttcaag aactgattaa tcggacgaga
451   ataaagatag agtcccgttc tacatgtcaa taccggcaac aatgaaattt
501   atagtaagag gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc
551   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	560	541	2.52e-08

Match details