JRC GMO-Amplicons | EURL GMFF

Record ID:	CP101147.1
Database:	nt	Go to original record
Description:	Oryza sativa Japonica Group cultivar Changxianggeng 1813 chromosome 7.
Taxon:	Oryza sativa Japonica Group
Original size:	43598337 bp

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Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	9693780	9694392	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggcaacttc caaattcaga gaaaccctgg aattaaaaaa
101   ggggcaatcc tgagccaaat ccatgttttg agaaaacaag cggttctcga
151   actagaaccc aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ctagcggaac tcccttctaa
251   attagggaaa gaagggcttt cgaaatctaa tacacacgta tagatactgg
301   catagcaaac gattaatcac agaactcata tcataatata ggttctttaa
351   ttctttttaa aatgaaaata ggaatgatta tgaaatagaa aattcataat
401   ttttttagaa ttgttgtgaa tccattccaa tcgaatattg agtaatcaaa
451   tccttcaatt catagttttc gaaatctttt aaaagcggat taatcggacg
501   aggataaaga gagagtccca ttctacatgt caatactgac aacaatgaaa
551   tttctagtaa aaggaaaatc cgtcgacttt ctaagtcgtg agggttcaag
601   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details