JRC GMO-Amplicons | EURL GMFF

Record ID:	OP271832.1
Database:	nt	Go to original record
Description:	Schnabelia terniflora chloroplast, complete genome.
Taxon:	Schnabelia terniflora
Original size:	156986 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47959	48509	+	551 bp

Amplicon:

551 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggtaat cctgagccaa atcctttttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aaaaaaaagg gtaggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttgactgc gttggtagag gaatctttcc acggaaactt
251   cagaaaggat gaaggataac cgtatctatt gaatactata tcaaatgatt
301   catgatgacc cgaatctcta ttttttagaa gaattggtgt gaattgattt
351   cacattgaag aaaaaaattg aatattcatt catcaaatca ttcactccac
401   agtccgatag atctttgaaa gaattgatta atcggacgag aataaagata
451   gagtcccgtt ctacatgtca ataccggcaa caatgaaatt tatagtaaga
501   ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc cctctatccc
551   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	551	532	2.48e-08

Match details