JRC GMO-Amplicons | EURL GMFF

Record ID:	OM735566.1
Database:	nt	Go to original record
Description:	Koenigia forrestii voucher Tsui1425 chloroplast, complete genome.
Taxon:	Koenigia forrestii
Original size:	156771 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47134	47718	+	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttaactg ttttgagcct tagtatggaa
51    acctactaag tgataatttt aaaattcaga gaaaccctgg aattaaaaaa
101   atgggcaatc ctgagccaac tcctgctttc caaaagtaag aataaaaaac
151   ggataggtgc agagactcaa tggaagctgt tctaacaaat ggagttgact
201   ctcaggcatt gtgatatgtt atatgaattc ttccatataa acttaaaaag
251   ggatgaagaa gaaacctata ggcatacgta cttaattata aatactatta
301   ttataaatag aatattgatg acgacccgaa tctttatttt atgaatatga
351   acaaataaaa taattattat gaatcgattt caagttgaag aaataatcta
401   atattcgttt cttaaaataa agtatttact ccacagtctg atagatcttt
451   tgaagaacca acccgttgga tgagaatgaa gatagagtcc cattctacgt
501   gtcaatctcg acaacaatga aatttatagt aagaggaaaa tccgtcgatt
551   ttaaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details