JRC GMO-Amplicons | EURL GMFF

Record ID:	OM457823.1
Database:	nt	Go to original record
Description:	Agastache rugosa chloroplast, complete genome.
Taxon:	Agastache rugosa
Original size:	151652 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46505	47062	+	558 bp

Amplicon:

558 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg actgcgccgg tagaggaatc tttccatgga aattttagaa
251   aggatgaagg ataaacgcat ctattgaata ctatatcaaa tgattaatgt
301   tggcccaaat ctgtttttct aatataaaaa tgaaaaaatc ggtgtgaatt
351   tatttcacgt tgaagaaaaa atagaatatt catcaactca ttcactccat
401   agtccggtag atcttttaaa gaactgatta atcggacgag aataaagata
451   gagtcccatt ctacatgcta catgtcaata ccggcaacaa tgaaatttat
501   agtaagagga aaatccgtcg actttaaaaa tcgtgagggt tcaagtccct
551   ctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	558	539	2.51e-08

Match details